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preimmune rabbit serum  (Millipore)


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    Millipore preimmune rabbit serum
    Preimmune Rabbit Serum, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+preimmune+serum/preimmune+rabbit+igg+antibody/pmc05024579-484-46-49
    Average 90 stars, based on 1 article reviews
    preimmune rabbit serum - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Negative Control:

    Article Title: Stargazin and Other Transmembrane AMPA Receptor Regulating Proteins Interact with Synaptic Scaffolding Protein MAGI-2 in Brain
    Article Snippet: The precleared brain extract was then incubated with 5 g of anti-stargazin (Upstate Biotechnology) antibodies for 1 h at 4°C, and then 50 l of anti-rabbit IgG beads was added for the next 2 h of incubation. .. Rabbit preimmune serum (Sigma) was used in place of antibodies for the negative control. ..

    Article Title: Stargazin and Other Transmembrane AMPA Receptor Regulating Proteins Interact with Synaptic Scaffolding Protein MAGI-2 in Brain
    Article Snippet: The precleared brain extract was then incubated with 5 μg of anti-stargazin (Upstate Biotechnology) antibodies for 1 h at 4°C, and then 50 μl of anti-rabbit IgG beads was added for the next 2 h of incubation. .. Rabbit preimmune serum (Sigma) was used in place of antibodies for the negative control. ..

    Control:

    Article Title: Sugarcane glycoproteins may act as signals for the production of xanthan in the plant-associated bacterium Xanthomonas albilineans
    Article Snippet: Sections were visualized in an Olympus BX 51 microscope. .. In each IGL preparation, a control to recognize non-specific binding of the gold-conjugated antibody was run in parallel, substituting the anti- X. albilineans primary antibody with a rabbit preimmune serum (Sigma-Aldrich Inc.). ..

    Binding Assay:

    Article Title: Sugarcane glycoproteins may act as signals for the production of xanthan in the plant-associated bacterium Xanthomonas albilineans
    Article Snippet: Sections were visualized in an Olympus BX 51 microscope. .. In each IGL preparation, a control to recognize non-specific binding of the gold-conjugated antibody was run in parallel, substituting the anti- X. albilineans primary antibody with a rabbit preimmune serum (Sigma-Aldrich Inc.). ..

    Incubation:

    Article Title: Cultured smooth muscle cells of the human vesical sphincter are more sensitive to histamine than are detrusor smooth muscle cells.
    Article Snippet: bjectives.. To compare histamine receptor expression in cultured smooth muscle cells from the human etrusor and internal sphincter using receptor-specific agonists. ethods.. Smooth muscle cells from the bladder dome and internal sphincter were cultured from 5 male atients undergoing cystectomy for bladder cancer therapy.

    other:

    Article Title: Inhibition of vasodilator neurotransmission in the sheep middle cerebral artery by VIP antiserum.
    Article Snippet: 1 Middle cerebral artery rings from the sheep were relaxed by vasoactive intestinal peptide (VIP) (20±200 nM) or by stimulation of the vasodilator nerves, electrical ®eld stimulation (EFS).. 2 VIP antiserum 1:256 inhibited the relaxation produced by both exogenous VIP (from 70+ 5 to 32+ 9% of 5-HT-induced tone at 200 nM VIP) and by EFS (from 43+ 5 to 26+ 6% of 5-HTinduced tone after 20 min), while pre-immune serum was inactive.. 3 Capsaicin (1 mM) produced a transient relaxation but did not alter the response to EFS which was subsequently inhibited by addition of L-NOArg (100 mM).



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    I. ricinus TROSPA protein-coated ELISA microplates were incubated with the serial dilutions of rabbit TROSPA antiserum or rabbit <t>preimmune</t> antiserum. After washing, the plates were treated with B. garinii OspA protein (C = 30 µg/ml, 100 µl/well), which was subsequently detected by FITC-conjugated anti -Borrelia goat polyclonal IgG. The X axis represents serial sera dilutions, and the Y axis represents the level of fluorescence counts. The level of TROSPA-OspA binding was determined for ten rabbit TROSPA antiserum or preimmune serum concentrations. The level of OspA binding was determined as the arithmetic mean of fluorescence counts based on 16 reactions performed for every TROSPA antiserum or preimmune serum dilution (for details see Materials and methods). The reduction of fluorescence counts was statistically significant (p<0,01) for all serum dilutions except the highest one.
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    I. ricinus TROSPA protein-coated ELISA microplates were incubated with the serial dilutions of rabbit TROSPA antiserum or rabbit <t>preimmune</t> antiserum. After washing, the plates were treated with B. garinii OspA protein (C = 30 µg/ml, 100 µl/well), which was subsequently detected by FITC-conjugated anti -Borrelia goat polyclonal IgG. The X axis represents serial sera dilutions, and the Y axis represents the level of fluorescence counts. The level of TROSPA-OspA binding was determined for ten rabbit TROSPA antiserum or preimmune serum concentrations. The level of OspA binding was determined as the arithmetic mean of fluorescence counts based on 16 reactions performed for every TROSPA antiserum or preimmune serum dilution (for details see Materials and methods). The reduction of fluorescence counts was statistically significant (p<0,01) for all serum dilutions except the highest one.
    Preimmune Rabbit Serum, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 1 article reviews
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    Image Search Results


    I. ricinus TROSPA protein-coated ELISA microplates were incubated with the serial dilutions of rabbit TROSPA antiserum or rabbit preimmune antiserum. After washing, the plates were treated with B. garinii OspA protein (C = 30 µg/ml, 100 µl/well), which was subsequently detected by FITC-conjugated anti -Borrelia goat polyclonal IgG. The X axis represents serial sera dilutions, and the Y axis represents the level of fluorescence counts. The level of TROSPA-OspA binding was determined for ten rabbit TROSPA antiserum or preimmune serum concentrations. The level of OspA binding was determined as the arithmetic mean of fluorescence counts based on 16 reactions performed for every TROSPA antiserum or preimmune serum dilution (for details see Materials and methods). The reduction of fluorescence counts was statistically significant (p<0,01) for all serum dilutions except the highest one.

    Journal: PLoS ONE

    Article Title: Functional Insights into Recombinant TROSPA Protein from Ixodes ricinus

    doi: 10.1371/journal.pone.0076848

    Figure Lengend Snippet: I. ricinus TROSPA protein-coated ELISA microplates were incubated with the serial dilutions of rabbit TROSPA antiserum or rabbit preimmune antiserum. After washing, the plates were treated with B. garinii OspA protein (C = 30 µg/ml, 100 µl/well), which was subsequently detected by FITC-conjugated anti -Borrelia goat polyclonal IgG. The X axis represents serial sera dilutions, and the Y axis represents the level of fluorescence counts. The level of TROSPA-OspA binding was determined for ten rabbit TROSPA antiserum or preimmune serum concentrations. The level of OspA binding was determined as the arithmetic mean of fluorescence counts based on 16 reactions performed for every TROSPA antiserum or preimmune serum dilution (for details see Materials and methods). The reduction of fluorescence counts was statistically significant (p<0,01) for all serum dilutions except the highest one.

    Article Snippet: TROSPA-coated ELISA microplates (prepared as described above) were incubated for 90 min at room temperature with 100 µl/well serially diluted (in PBST) rabbit TROSPA antiserum or rabbit preimmune serum (Eurogentec).

    Techniques: Enzyme-linked Immunosorbent Assay, Incubation, Fluorescence, Binding Assay